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CSEC>> Biology

SBA guidelines (cont'd)
Monacia Williams, Contributor

Last week, we looked at a sample mark scheme for an experiment used to test the skills for ORR (observation, reporting, recording). I hope you learned something that will help you in writing up your own experiments. This week, we will look at a written example that would give you 10 marks based on that mark scheme.

Remember, all written experiments require certain common headings. These are: Date, Topic, Aim, Apparatus/Materials, Method, Results, Discussion and Conclusion. For ORR, having all of these headings written in the correct order will get you a mark. Let us see how closely your written experiments come to this one. Remember also that all experiments must be written in reported speech or the past tense. You are giving a report of work that you have done, not work that you are going to do or are still doing!

Date: This is the day on which the experiment was done.

Topic: Enzymes

Aim: To investigate the effect of pH on enzyme activity.

Apparatus/Materials: Pill bottles, paper circles, beaker, forceps, petri dish, stopwatch, potato extract, hydrogen peroxide, buffer solutions of pH 3, 4, 5, 6, 7 and 8.

Method:

1. 50 cm2 of each buffer solution was placed into pill bottles labelled pH 3, 4, 5 6, 7 and 8.

2. 10 cm3 of hydrogen peroxide were added to each pill bottle.

3. 20 circles of paper, made using a paper punch, were collected in a dry pill bottle.

4. 10 cm2 of potato extract were collected in another pill bottle.

5. A single circle of paper was dipped into the extract and left for 10 seconds.

6. The forceps were used to remove the paper circle and to place it into the pill bottle labelled pH 3.

7. As the paper was placed into the solution the stopwatch was started. As soon as the paper rose to the top of the solution the watch was stopped.

8. The time was recorded in a table; this was done twice more.

9. Steps 5 to 8 were repeated for each solution. The average time taken for the paper to rise in each solution was calculated.

10. The information recorded in the table was used to plot a graph of rate of reaction against pH.

Results: Table showing the effect of changing pH on the action of the enzyme catalase

Graph showing the effect of changing pH on the action of the enzyme catalase

Another thing that you must always note is that you should not draw a graph without first creating a table. The table holds the information that you need for the graph!

Guess what? This is all we have time for this week. Next week, we will continue with the discussion and conclusion for this experiment. Until then, have a wonderful week!

Monacia Williams teaches at Glenmuir High School. Send questions and comments to kerry-ann.hepburn@gleanerjm.com


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